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Open Access
Copyright: The author. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original Research
4.
Histopathological effects of
2,4-dichlorophenoxyacetic acid on sprague-dawley
rats with special reference to its possible
carcinogenicity -
Khaled M A Hassanein
Vet World. 2012; 5(1): 24-30
doi:
10.5455/vetworld.201 2.24-30
Abstract
This study investigated the toxic effects of
2,4-dichlorophenoxyacetic acid on male Sprague-Dawley
rats. In which, 30 rats were divided into three
even groups. First group received orally normal
saline (3 ml/kg b.w.) and kept as normal control
in parallel to treated groups (5 rats each),
second group received orally
2,4-dichloropheno-xyacetic acid (30 mg/kg b.w.)
for 2 months and third group received orally
2,4-dichlorophenoxyacetic acid (30 mg/kg b.w.) for
6 months. At the end of each treatment, specimens
from the liver, kidneys, lungs, testicles,
epididymis and brain were obtained and subjected
for histopathological examination. After 2 months
of treatment with 2,4-dichlorophenoxyacetic acid,
the overall changes were milder and ranged from
vascular to necrobiotic findings. On the other
hand, changes seen after 6 months were severe
particularly in the liver and kidney. In the
liver, there were focal areas of necrosis and
preneoplastic changes in the form eosinophilic
preneoplastic foci, oval cell proliferation and
spongiotic pericytoma. Kidneys showed glomerular
swelling, thickening of the glomerular basement
membrane and proliferation of the renal tubular
epithelium. Lesions in the other organs included
necrosis and sloughing of bronchiolar epithelial
lining and mononuclear cellular infiltration in
the lungs, necrosis of seminiferous tubular
epithelium in the testicles and neuronal
degeneration in the brain. In conclusion,
2,4-dichlorophenoxyacetic acid induced deleterious
pathological effects on the vital organs including
preneoplastic changes in the liver in Sprague-Dawley
rats. Keywords: 2,4-dichlorophenoxyacetic
acid, herbicides, histopathology, preneoplasia,
Sprague-Dawley rats.
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